Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Dot Products

Abstract:
Analytical Applications of Enzymatic Growth of Quantum Dots

Dot Products

Weinheim, Germany | Posted on June 16th, 2010

The search for time and cost effective as well as sensitive methods for bioanalytical assays is currently of great interest. At the center of Biofunctional Nanomaterials in San Sebastian (Spain), Valery Pavlov and his co-workers are undertaking research in the area of the design and preparation of new biomaterials for such applications. They report on a new analytical approach in which the enzymatic generation of quantum dots can be applied to the detection of enzymatic activities in Chemistry—A European Journal.

Bioanalytical assays based on the enzymatic generation of nanoparticles are not very common and mostly are limited to biochemical growth of nonfluorescent metal nanoparticles. The main drawback of such systems is that only UV/Vis spectroscopy can be utilized for the optical reading of a signal. Fluorescence spectroscopy, on the other hand, provides an alternative and more sensitive method of analysis.

Valery and and co-workers utilise the fact that alkaline phosphatase (ALP) can induce the formation of H2S. In the presence of cadmium cations, H2S reacts to yield CdS quantum dots that show a characteristic fluorescence, dependent on the concentration of enzyme or substrate. The formation of CdS quantum dots can be measured by fluorescence spectroscopy and represents an alternative to other analytical methods.

ALP is an enzyme that removes phosphate groups from biomolecules, such as alkaloids, proteins and nucleotides and finds wide application in bioanalysis. This enzyme is broadly used as a label in enzyme-linked immunosorbent assays (ELISA). Another important application of ALP is the monitoring of pasteurization in cows' milk—well-pasteurized milk should not demonstrate any phosphatase activity due to enzyme deactivation at elevated temperature. In addition the measurement of ALP activity in human blood serum is used for the diagnostics of viral acute and chronic hepatitis and cirrhosis. The disadvantages of the current methods include high costs, special storage conditions, and maximum fluorescence of the reaction product at alkaline pH. This new assay based on the enzymatic generation of quantum dots and detection by fluorescence spectroscopy represents a step in the direction of finding a method that can significantly reduce time and costs related with these important tests.

Author: Valery Pavlov, CIC biomaGUNE, San Sebastian (Spain), www.cicbiomagune.es/

Title: Analytical Applications of Enzymatic Growth of Quantum Dots

Chemistry - A European Journal 2010, 16, No. 21, 6187-6192, Permalink to the article: dx.doi.org/10.1002/chem.200903373

####

For more information, please click here

Copyright © Chemistry - A European Journal

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Researchers are cracking the code on solid-state batteries: Using a combination of advanced imagery and ultra-thin coatings, University of Missouri researchers are working to revolutionize solid-state battery performance February 28th, 2025

Unraveling the origin of extremely bright quantum emitters: Researchers from Osaka University have discovered the fundamental properties of single-photon emitters at an oxide/semiconductor interface, which could be crucial for scalable quantum technology February 28th, 2025

Closing the gaps — MXene-coating filters can enhance performance and reusability February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

Academic/Education

Rice University launches Rice Synthetic Biology Institute to improve lives January 12th, 2024

Multi-institution, $4.6 million NSF grant to fund nanotechnology training September 9th, 2022

National Space Society Helps Fund Expanding Frontier’s Brownsville Summer Entrepreneur Academy: National Space Society and Club for the Future to Support Youth Development Program in South Texas June 24th, 2022

How a physicist aims to reduce the noise in quantum computing: NAU assistant professor Ryan Behunin received an NSF CAREER grant to study how to reduce the noise produced in the process of quantum computing, which will make it better and more practical April 1st, 2022

Nanomedicine

Multiphoton polymerization: A promising technology for precision medicine February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

SMART researchers pioneer first-of-its-kind nanosensor for real-time iron detection in plants February 28th, 2025

How a milk component could eliminate one of the biggest challenges in treating cancer and other disease, including rare diseases: Nebraska startup to use nanoparticles found in milk to target therapeutics to specific cells January 17th, 2025

Sensors

Quantum engineers ‘squeeze’ laser frequency combs to make more sensitive gas sensors January 17th, 2025

UCF researcher discovers new technique for infrared “color” detection and imaging: The new specialized tunable detection and imaging technique for infrared photons surpasses present technology and may be a cost-effective method of capturing thermal imaging or night vision, medica December 13th, 2024

Beyond wires: Bubble technology powers next-generation electronics:New laser-based bubble printing technique creates ultra-flexible liquid metal circuits November 8th, 2024

Nanotechnology: Flexible biosensors with modular design November 8th, 2024

Announcements

Development of 'transparent stretchable substrate' without image distortion could revolutionize next-generation displays Overcoming: Poisson's ratio enables fully transparent, distortion-free, non-deformable display substrates February 28th, 2025

Unraveling the origin of extremely bright quantum emitters: Researchers from Osaka University have discovered the fundamental properties of single-photon emitters at an oxide/semiconductor interface, which could be crucial for scalable quantum technology February 28th, 2025

Closing the gaps — MXene-coating filters can enhance performance and reusability February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

Quantum Dots/Rods

A new kind of magnetism November 17th, 2023

IOP Publishing celebrates World Quantum Day with the announcement of a special quantum collection and the winners of two prestigious quantum awards April 14th, 2023

Qubits on strong stimulants: Researchers find ways to improve the storage time of quantum information in a spin rich material January 27th, 2023

NIST’s grid of quantum islands could reveal secrets for powerful technologies November 18th, 2022

Nanobiotechnology

Multiphoton polymerization: A promising technology for precision medicine February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

SMART researchers pioneer first-of-its-kind nanosensor for real-time iron detection in plants February 28th, 2025

How a milk component could eliminate one of the biggest challenges in treating cancer and other disease, including rare diseases: Nebraska startup to use nanoparticles found in milk to target therapeutics to specific cells January 17th, 2025

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project