Nanotechnology Now - Press Release: News from Quorum: Diamond Light Source use Quorum Cryo-SEM preparation system in conjunction with a new beamline

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The image above illustrates the data quality we get routinely — crystals of cytoplasmic polyhedrosis virus (CPV20). The crystals shown here are in the size range 2.5 to 5 µm.”

Abstract:
Quorum Technologies, market and technology leaders in electron microscopy coating and cryogenic preparation products, report on the use of their PP3000T cryo preparation system in conjunction with the new beamline at Diamond Light Source to help align protein crystals prior to X-ray macromolecular crystallography experiments.

News from Quorum: Diamond Light Source use Quorum Cryo-SEM preparation system in conjunction with a new beamline

Laughton, UK | Posted on July 5th, 2016

Dr Anna Warren is a Senior Support Scientist on VMXm, a new beamline currently under development and construction at Diamond Light Source where it will be used to carry out X-ray macromolecular crystallography experiments. Diamond offers 26 separate beamlines to aid with research groups in the UK and abroad, allowing them to determine the three dimensional structure of matter, such as protein molecules, on the atomic scale. This can help to aid with drug design and to assist in the understanding of how these proteins function. There are several beamlines already running at Diamond which allow the study of protein crystals ranging from 5-100 microns in size. However, as the systems get more complicated, inherently the crystals get smaller, so the beamline currently being built will allow the study of crystals ~500 nm to 5 microns in size.

Due to the small size of these crystals, Dr Warren is using a JEOL SEM to visualise the crystals and align them to the X-ray beam to carry out the experiments. The crystals are very fragile and prone to dehydration. This means they have to be stored, handled and studied at liquid nitrogen temperatures. This is where the Quorum PP3000T cryo-SEM preparation system has been of great use. Dr Warren takes up the story: “It has allowed us to visualise crystals less than a micron in size at cryogenic temperatures. This technique has never been used in our field in this way so we are currently carrying out a lot of research to understand the best ways of handling and preparing samples to obtain the best quality images. This would be impossible without having the Quorum preparation system and cryo-stage as the crystals would deteriorate in quality. Quorum was very helpful when we started looking at systems to purchase, especially as they have an SEM and cryo-SEM set up in Laughton. We were able to test out our samples in advance to get an understanding of whether it would work for us. Since making our purchase decision, we found that the preparation desk/slushing station have become a vital part of our sample preparation and this has been invaluable for our experiments.

The Quorum PP3010T and PP3000T are highly automated, easy to use, column-mounted, gas-cooled cryo preparation systems suitable for most makes and models of SEM, FE-SEM and FIB/SEM. Highly efficient cooling allows temperatures of down to 190 °C to be routinely achieved. The systems include facilities for rapid freezing, cold fracturing, controlled sublimation and high resolution sputter coating. The typical process time for samples is between five and ten minutes.

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About Quorum Technologies Limited
Quorum Technologies are market and technology leaders in electron microscopy coating and cryogenic preparation. The company was founded in 2001 after the acquisition of the long-established Polaron brand and its range of EM products from Thermo Instruments. In 2005, they acquired the Emitech company allowing Quorum to extend their product range and to further grow the business.

Since the start, Quorum has been responding to industry requests and requirements for new instruments and techniques. This first culminated in the introduction of the market-leading Q Series of vacuum coaters and PP3000T/PP3010T cryo preparation systems for SEM and FIB/SEM - both true ground-up redesigns that have truly set the standard within the specimen preparation field. Further impetus and investment came with the acquisition of Quorum by Judges Scientific plc and a move to a purpose-built factory and offices close to the South Downs National Park at Laughton, East Sussex in the South East of England.

In 2014 Quorum were proud winners of the Queen's Award for Enterprise for International Trade.

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www.quorumtech.com

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