Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Development of Gold Nanoparticles That Control Osteogenic Differentiation of Stem Cells

Human mesenchymal stem cells (hMSCs) resulted from the following steps: each type of gold nanoparticles was added to hMSCs, and the treated cells were cultured for 3 weeks. The cells received either alkaline phosphatase (ALP) staining or alizarin red staining (ARS) of calcium phosphate deposits, both of which are osteogenic differentiation indicators. Control experiments with unmodified gold nanoparticles and untreated hMSCs were also carried out for comparison. In the upper images, ALP positive cells were stained purple while agglomerates of gold nanoparticles were detected as blue dots. In the lower images, spider-web-like red stains represent calcium phosphate deposits while bluish purple dots indicate agglomerates of gold nanoparticles. All scale bars are 500 μm.
Human mesenchymal stem cells (hMSCs) resulted from the following steps: each type of gold nanoparticles was added to hMSCs, and the treated cells were cultured for 3 weeks. The cells received either alkaline phosphatase (ALP) staining or alizarin red staining (ARS) of calcium phosphate deposits, both of which are osteogenic differentiation indicators. Control experiments with unmodified gold nanoparticles and untreated hMSCs were also carried out for comparison. In the upper images, ALP positive cells were stained purple while agglomerates of gold nanoparticles were detected as blue dots. In the lower images, spider-web-like red stains represent calcium phosphate deposits while bluish purple dots indicate agglomerates of gold nanoparticles. All scale bars are 500 μm.

Abstract:
Tissue Regeneration Materials Unit (Guoping Chen, Unit Director) at the International Center for Materials Nanoarchitectonics (MANA) (Masakazu Aono, Director General, MANA), NIMS (Sukekatsu Ushioda, President) successfully developed gold nanoparticles that have functional surfaces and act on osteogenic differentiation of stem cells.

Development of Gold Nanoparticles That Control Osteogenic Differentiation of Stem Cells

Tsukuba, Japan | Posted on June 9th, 2015

In regenerative medicine, the technology to control stem cell functions such as differentiation and proliferation is indispensable. It has been reported that nanosized gold particles promote the differentiation of human mesenchymal stem cells into osteoblasts. Also, other studies suggested that various functional groups such as amino, carboxyl and hydroxyl groups promote or inhibit stem cell differentiation. Based on these reports, we assumed that gold nanoparticles with surface modified with functional groups is a promising candidate to control stem cell functions. However, specific effects of such particles on the differentiation of human mesenchymal stem cells was unknown.

We synthesized gold nanoparticles with surface modified with one of the following functional groups: a positively-charged amino group (-NH2), a negatively-charged carboxyl group (-COOH) or a neutral hydroxyl group (-OH), and identified how they affect the osteogenic differentiation of mesenchymal stem cells that were derived from human bone marrow. Among these three types of nanoparticles, those with the carboxyl groups were uptaken by cells and exhibited a strong bone differentiation-inhibitory effect compared to the other types of nanoparticles. Furthermore, we investigated the effect of gold nanoparticles with carboxyl groups on the gene expression profile of mesenchymal stem cell from human bone marrow. The results indicated that the nanoparticles inhibited several gene expressions related to osteogenic differentiation. Therefore, the influence of the gold nanoparticles on promoting or inhibiting osteogenic differentiation varied depending on the types of functional groups.

In view of regenerative medicine, it is essential to develop technology enabling controlling stem cell functions as well as safe and high-quality stem cells. In the present study, we attempted to control stem cell functions through material manipulation, and our findings will contribute to the creation of novel nanomaterials that facilitate the advancement of stem cell manipulation. We intend to build upon these results in our future endeavors in developing regenerative medicine.

####

About National Institute for Materials Science (NIMS)
Public research institution for materials science in Japan.

For more information, please click here

Contacts:
(Regarding this research)
Guoping Chen, Unit Director
Tissue Regeneration Materials Unit
International Center for Materials Nanoarchitectonics
National Institute for Materials Science

Tel: 029-860-4496

(Regarding public relations)
Public Relations Office
National Institute for Materials Science
1-2-1 Sengen, Tsukuba, Ibaraki, 305-0047, JAPAN
Tel: 029-859-2026, Fax: 029-859-2017

Copyright © AlphaGalileo

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Full bibliographic information

Related News Press

News and information

Researchers are cracking the code on solid-state batteries: Using a combination of advanced imagery and ultra-thin coatings, University of Missouri researchers are working to revolutionize solid-state battery performance February 28th, 2025

Unraveling the origin of extremely bright quantum emitters: Researchers from Osaka University have discovered the fundamental properties of single-photon emitters at an oxide/semiconductor interface, which could be crucial for scalable quantum technology February 28th, 2025

Closing the gaps — MXene-coating filters can enhance performance and reusability February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

Laboratories

Giving batteries a longer life with the Advanced Photon Source: New research uncovers a hydrogen-centered mechanism that triggers degradation in the lithium-ion batteries that power electric vehicles September 13th, 2024

A 2D device for quantum cooling:EPFL engineers have created a device that can efficiently convert heat into electrical voltage at temperatures lower than that of outer space. The innovation could help overcome a significant obstacle to the advancement of quantum computing technol July 5th, 2024

A battery’s hopping ions remember where they’ve been: Seen in atomic detail, the seemingly smooth flow of ions through a battery’s electrolyte is surprisingly complicated February 16th, 2024

NRL discovers two-dimensional waveguides February 16th, 2024

Govt.-Legislation/Regulation/Funding/Policy

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

Department of Energy announces $71 million for research on quantum information science enabled discoveries in high energy physics: Projects combine theory and experiment to open new windows on the universe January 17th, 2025

Quantum engineers ‘squeeze’ laser frequency combs to make more sensitive gas sensors January 17th, 2025

Chainmail-like material could be the future of armor: First 2D mechanically interlocked polymer exhibits exceptional flexibility and strength January 17th, 2025

Nanomedicine

Multiphoton polymerization: A promising technology for precision medicine February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

SMART researchers pioneer first-of-its-kind nanosensor for real-time iron detection in plants February 28th, 2025

How a milk component could eliminate one of the biggest challenges in treating cancer and other disease, including rare diseases: Nebraska startup to use nanoparticles found in milk to target therapeutics to specific cells January 17th, 2025

Discoveries

Development of 'transparent stretchable substrate' without image distortion could revolutionize next-generation displays Overcoming: Poisson's ratio enables fully transparent, distortion-free, non-deformable display substrates February 28th, 2025

Unraveling the origin of extremely bright quantum emitters: Researchers from Osaka University have discovered the fundamental properties of single-photon emitters at an oxide/semiconductor interface, which could be crucial for scalable quantum technology February 28th, 2025

Closing the gaps — MXene-coating filters can enhance performance and reusability February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

Materials/Metamaterials/Magnetoresistance

Chainmail-like material could be the future of armor: First 2D mechanically interlocked polymer exhibits exceptional flexibility and strength January 17th, 2025

Enhancing transverse thermoelectric conversion performance in magnetic materials with tilted structural design: A new approach to developing practical thermoelectric technologies December 13th, 2024

FSU researchers develop new methods to generate and improve magnetism of 2D materials December 13th, 2024

New material to make next generation of electronics faster and more efficient With the increase of new technology and artificial intelligence, the demand for efficient and powerful semiconductors continues to grow November 8th, 2024

Announcements

Development of 'transparent stretchable substrate' without image distortion could revolutionize next-generation displays Overcoming: Poisson's ratio enables fully transparent, distortion-free, non-deformable display substrates February 28th, 2025

Unraveling the origin of extremely bright quantum emitters: Researchers from Osaka University have discovered the fundamental properties of single-photon emitters at an oxide/semiconductor interface, which could be crucial for scalable quantum technology February 28th, 2025

Closing the gaps — MXene-coating filters can enhance performance and reusability February 28th, 2025

Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers/Posters

Development of 'transparent stretchable substrate' without image distortion could revolutionize next-generation displays Overcoming: Poisson's ratio enables fully transparent, distortion-free, non-deformable display substrates February 28th, 2025

Leading the charge to better batteries February 28th, 2025

Quantum interference in molecule-surface collisions February 28th, 2025

New ocelot chip makes strides in quantum computing: Based on "cat qubits," the technology provides a new way to reduce quantum errors February 28th, 2025

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project