Home > Press > Iowa State, Ames Lab researchers invent new tool to study single biological molecules
![]() |
Iowa State University and Ames Laboratory researchers, left to right, Sanjeevi Sivasankar, Chi-Fu Yen and Hui Li have invented microscope technology to study single biological molecules. Larger photo. Photo by Bob Elbert. |
Abstract:
By blending optical and atomic force microscope technologies, Iowa State University and Ames Laboratory researchers have found a way to complete 3-D measurements of single biological molecules with unprecedented accuracy and precision.
Existing technologies allow researchers to measure single molecules on the x and y axes of a 2-D plane. The new technology allows researchers to make height measurements (the z axis) down to the nanometer - just a billionth of a meter - without custom optics or special surfaces for the samples.
"This is a completely new type of measurement that can be used to determine the z position of molecules," said Sanjeevi Sivasankar, an Iowa State assistant professor of physics and astronomy and an associate of the U.S. Department of Energy's Ames Laboratory.
Details of the technology were recently published by the journal Nano Letters. Co-authors of the study are Sivasankar; Hui Li, an Iowa State post-doctoral research associate in physics and astronomy and an associate of the Ames Laboratory; and Chi-Fu Yen, an Iowa State doctoral student in electrical and computer engineering and a student associate of the Ames Laboratory.
The project was supported by lab startup funds from Iowa State University and a $120,075 grant from the Grow Iowa Values Fund, a state economic development program.
Sivasankar's research program has two objectives: to learn how biological cells adhere to each other and to develop new tools to study those cells.
That's why the new microscope technology - called standing wave axial nanometry (SWAN) - was developed in Sivasankar's lab.
Here's how the technology works: Researchers attach a commercial atomic force microscope to a single molecule fluorescence microscope. The tip of the atomic force microscope is positioned over a focused laser beam, creating a standing wave pattern. A molecule that has been treated to emit light is placed within the standing wave. As the tip of the atomic force microscope moves up and down, the fluorescence emitted by the molecule fluctuates in a way that corresponds to its distance from the surface. That distance can be compared to a marker on the surface and measured.
"We can detect the height of the molecule with nanometer accuracy and precision," Sivasankar said.
The paper reports that measurements of a molecule's height are accurate to less than a nanometer. It also reports that measurements can be taken again and again to a precision of 3.7 nanometers.
Sivasankar's research team used fluorescent nanospheres and single strands of DNA to calibrate, test and prove their new instrument.
Users who could benefit from the technology include medical researchers who need high-resolution data from microscopes. Sivasankar thinks the technology has commercial potential and is confident it will advance his own work in single molecule biophysics.
"We hope to use this technology to move that research forward," he said. "And in doing that, we'll continue to invent new technologies."
####
For more information, please click here
Contacts:
Sanjeevi Sivasankar
515-294-1220
Copyright © Iowa State University
If you have a comment, please Contact us.Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.
Related News Press |
News and information
Electrifying results shed light on graphene foam as a potential material for lab grown cartilage June 6th, 2025
Quantum computers simulate fundamental physics: shedding light on the building blocks of nature June 6th, 2025
A 1960s idea inspires NBI researchers to study hitherto inaccessible quantum states June 6th, 2025
Imaging
Turning up the signal November 8th, 2024
New discovery aims to improve the design of microelectronic devices September 13th, 2024
Quantum researchers cause controlled ‘wobble’ in the nucleus of a single atom September 13th, 2024
Nanomedicine
Ben-Gurion University of the Negev researchers several steps closer to harnessing patient's own T-cells to fight off cancer June 6th, 2025
Cambridge chemists discover simple way to build bigger molecules – one carbon at a time June 6th, 2025
Electrifying results shed light on graphene foam as a potential material for lab grown cartilage June 6th, 2025
Self-propelled protein-based nanomotors for enhanced cancer therapy by inducing ferroptosis June 6th, 2025
Discoveries
Researchers unveil a groundbreaking clay-based solution to capture carbon dioxide and combat climate change June 6th, 2025
Cambridge chemists discover simple way to build bigger molecules – one carbon at a time June 6th, 2025
Electrifying results shed light on graphene foam as a potential material for lab grown cartilage June 6th, 2025
A 1960s idea inspires NBI researchers to study hitherto inaccessible quantum states June 6th, 2025
Announcements
Electrifying results shed light on graphene foam as a potential material for lab grown cartilage June 6th, 2025
Quantum computers simulate fundamental physics: shedding light on the building blocks of nature June 6th, 2025
A 1960s idea inspires NBI researchers to study hitherto inaccessible quantum states June 6th, 2025
Tools
Rice researchers harness gravity to create low-cost device for rapid cell analysis February 28th, 2025
New 2D multifractal tools delve into Pollock's expressionism January 17th, 2025
Nanobiotechnology
Ben-Gurion University of the Negev researchers several steps closer to harnessing patient's own T-cells to fight off cancer June 6th, 2025
Electrifying results shed light on graphene foam as a potential material for lab grown cartilage June 6th, 2025
Self-propelled protein-based nanomotors for enhanced cancer therapy by inducing ferroptosis June 6th, 2025
![]() |
||
![]() |
||
The latest news from around the world, FREE | ||
![]() |
![]() |
||
Premium Products | ||
![]() |
||
Only the news you want to read!
Learn More |
||
![]() |
||
Full-service, expert consulting
Learn More |
||
![]() |